RESUMO
OBJECTIVE: To screen the habitat processing technology of Isatis tinctoria slices, and provide basis for its quality.METHODS: Appearance score, the contents of moisture, ash, extract and main component (R, S) -goitrin in I. tinctoria slices prepared by 4 kinds of dried degree [A. 60 ℃ drying after cutting; B. cutting after natural drying; C. cutting after natural drying (13-15 d), drying at 60 ℃ again; D. cutting after natural drying (30-50 d), drying at 60 ℃ again]from fresh harvested sample. RESULTS: Appearance score was in descending order with C>D>A>B; the content of moisture was in descending order with B>A>D>C. There was little difference in the content of ash among different methods. The contents of extract and (R, S)-goitrin were in descending order with C>A>B>D. CONCLUSIONS: The quality of I. tinctoria slices prepared by 4 processing technologies are different, and the quality of I. tinctoria slices prepared by cutting after natural drying (about 15 d)and drying at 60 ℃ again is relatively best.
RESUMO
OBJECTIVE:To establish a preparative separation method for euphol reference substance in Euphorbia pekinensis. METHODS:Parts of petroleum ether extraction from E. pekinensis ethanol extract were separated by silica gel column chromatogra-phy with petroleum ether-ethyl acetate(95∶5-70∶30,V/V)by gradient elution. The enriched fractions of euphol were collected. With the methanol repeated recrystallization,nuclear magnetic resonance spectroscopy(NMR)method,mass spectrometry(MS)meth-od and other spectroscopic methods were used to identify the chemical structures,and thin layer chromatography (TLC),UV, HPLC-UV,HPLC-MS were combined to detect the mass fraction. RESULTS:The mass fraction of euphol reference substance sepa-rated from E. pekinensis was>99%. CONCLUSIONS:The reference substance of euphol acquired by this method meet the relative requirements of the chemical reference substance in the content dertermination of TCM new drug quality standard. It provides chemi-cal reference substance for the quality control of E. pekinensis and prescription preparations containing E. pekinensis and the basic research of effective substances.
RESUMO
<p><b>OBJECTIVE</b>To compare the crystal form with the toxicity intensitity of raphides in four poisonous herbs of Araceae family.</p><p><b>METHOD</b>The four kinds of raphides were extracted and isolated from Pinellia ternate, P. pedatisecta, Arisaema amurense and Typhonium giganteum. These raphides were observed with scanning electron microscopy (SEM), and the elements were analyzed with X-ray photoelectron spectroscopy. Infrared spectrum was used for detecting the functional groups. Toxic intensities of the four kinds of raphides were detected by mice acute toxicity experiment, and the value of LD0 were from caculated by Bliss rule.</p><p><b>RESULT</b>The raphides in the four plants have the similar crystal form. Observation with SEM showed a pointed and blunt end, and a long groove and barbs on a raphide. The raphides in P. ternate and P. pedatisecta were sharper than that in other two, respectively. The results of X-ray diffraction, photoelectric spectra showed that the major component of raphides was calcium oxalate monohydrate, and also showed the elements of N and S existing. Infrared spectra showed the raphides contained functional groups of -COOH and -NH2. These results illustrated that the calcium oxalate monohydrate was not the only component of the raphide. The raphides could produce severe toxic reactions. LD50 values of P. ternate, P. pedatisecta, A. amurense and T. giganteum were 14.78, 14.11, 16.02 and 18.90 mg x kg(-1) (ip), respectively. The corresponding LD50 values of crude drugs were all above 3000 mg x kg(-1) (ip). The toxicity of raphides was 200 times of crude drugs'.</p><p><b>CONCLUSION</b>The raphides in P. ternate and P. pedatisecta, A. amurense and T. giganteum were their common poisonous factor.</p>
